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normal human epidermal keratinocytes  (PromoCell)


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    PromoCell normal human epidermal keratinocytes
    Dose-dependent effects on cytokine secretion. <t>NHEKs</t> (p3–5) cultured to 80% confluency were irradiated at 17–33 mJ/cm². Supernatants were collected 24 h post-irradiation and assessed via cytokine array ( A ) or via ELISA for IL-6 ( B ), IL-8 ( C ), CCL20 ( D ), and TNF-α ( E ). ELISA data are presented as mean ± SD. **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.
    Normal Human Epidermal Keratinocytes, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+foreskin+keratinocytes/Normal+Human+Epidermal+Keratinocytes+(NHEK)+juvenile+foreskin%2C+single+donor/pmc13188905-44-5-13
    Average 95 stars, based on 42 article reviews
    normal human epidermal keratinocytes - by Bioz Stars, 2026-09
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    1) Product Images from "Skin Staphylococcus species differentially modulate keratinocyte cytokine secretion in response to UVB"

    Article Title: Skin Staphylococcus species differentially modulate keratinocyte cytokine secretion in response to UVB

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/aem.01549-25

    Dose-dependent effects on cytokine secretion. NHEKs (p3–5) cultured to 80% confluency were irradiated at 17–33 mJ/cm². Supernatants were collected 24 h post-irradiation and assessed via cytokine array ( A ) or via ELISA for IL-6 ( B ), IL-8 ( C ), CCL20 ( D ), and TNF-α ( E ). ELISA data are presented as mean ± SD. **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.
    Figure Legend Snippet: Dose-dependent effects on cytokine secretion. NHEKs (p3–5) cultured to 80% confluency were irradiated at 17–33 mJ/cm². Supernatants were collected 24 h post-irradiation and assessed via cytokine array ( A ) or via ELISA for IL-6 ( B ), IL-8 ( C ), CCL20 ( D ), and TNF-α ( E ). ELISA data are presented as mean ± SD. **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.

    Techniques Used: Cell Culture, Irradiation, Enzyme-linked Immunosorbent Assay

    Exposure to a skin PMC elevates cytokine secretion regardless of UVB exposure. NHEKs (p2–5) were treated with PBS (PBS, no UVB) or S. epidermidis, S. hominis, M. luteus, C. tuberculostearicum, and C. acnes for 30–90 min and subsequently exposed to UVB (33 mJ/cm²). Supernatants were collected 24 h post-inoculation with each organism, and the effects of the skin microbial PMC ± UVB on IL-6 ( A ), IL-8 ( B ), TNF-α ( C ), and CCL20 ( D ) secretion were assessed using ELISA. ** P < 0.01 and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.
    Figure Legend Snippet: Exposure to a skin PMC elevates cytokine secretion regardless of UVB exposure. NHEKs (p2–5) were treated with PBS (PBS, no UVB) or S. epidermidis, S. hominis, M. luteus, C. tuberculostearicum, and C. acnes for 30–90 min and subsequently exposed to UVB (33 mJ/cm²). Supernatants were collected 24 h post-inoculation with each organism, and the effects of the skin microbial PMC ± UVB on IL-6 ( A ), IL-8 ( B ), TNF-α ( C ), and CCL20 ( D ) secretion were assessed using ELISA. ** P < 0.01 and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    UVB impacts the relative abundance of skin commensals in a polymicrobial skin community. NHEKs (p2–5) were incubated with a polymicrobial community for 90 min, media replaced, and cultures exposed to 33 mJ/cm2 UVB or left unirradiated (no UVB). Polymicrobial community composition was assessed 24 h post-treatment via qPCR, where relative proportions of each organism were calculated via species-specific standard curves and presented as collated data ( A , results for C. tuberculostearicum and M. luteus are not visible in the graph, as abundance was <1% of the total community) and UVB induced changes on individual species within the community of S. epidermidis ( B ), S. hominis ( C ), C. acnes ( D ), M. luteus ( E ), and C. tuberculostearicum ( F ). Data are presented as mean ± SD. ** P < 0.01 and * P < 0.05, as determined via unpaired t -test.
    Figure Legend Snippet: UVB impacts the relative abundance of skin commensals in a polymicrobial skin community. NHEKs (p2–5) were incubated with a polymicrobial community for 90 min, media replaced, and cultures exposed to 33 mJ/cm2 UVB or left unirradiated (no UVB). Polymicrobial community composition was assessed 24 h post-treatment via qPCR, where relative proportions of each organism were calculated via species-specific standard curves and presented as collated data ( A , results for C. tuberculostearicum and M. luteus are not visible in the graph, as abundance was <1% of the total community) and UVB induced changes on individual species within the community of S. epidermidis ( B ), S. hominis ( C ), C. acnes ( D ), M. luteus ( E ), and C. tuberculostearicum ( F ). Data are presented as mean ± SD. ** P < 0.01 and * P < 0.05, as determined via unpaired t -test.

    Techniques Used: Incubation

    UVB exacerbates S. epidermidis -induced cytokine secretion, while S. hominis amplified secretion of IL-8 and CCL20 regardless of UVB exposure. NHEKs (p2–5) were incubated with media alone, S. hominis, or S. epidermidis for 30–60 min, media replaced with PBS, and exposed to UVB (0–33 mJ/cm²). Adherent bacteria were then co-cultured with NHEKs for 24 h, following which supernatants were collected to assess the effects on IL-6 ( A and E ), CCL20 ( B and F ), IL-8 ( C and G ), and TNF-α ( D and H ). Significance was assessed via one-way ANOVA followed by Tukey test, where ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
    Figure Legend Snippet: UVB exacerbates S. epidermidis -induced cytokine secretion, while S. hominis amplified secretion of IL-8 and CCL20 regardless of UVB exposure. NHEKs (p2–5) were incubated with media alone, S. hominis, or S. epidermidis for 30–60 min, media replaced with PBS, and exposed to UVB (0–33 mJ/cm²). Adherent bacteria were then co-cultured with NHEKs for 24 h, following which supernatants were collected to assess the effects on IL-6 ( A and E ), CCL20 ( B and F ), IL-8 ( C and G ), and TNF-α ( D and H ). Significance was assessed via one-way ANOVA followed by Tukey test, where ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

    Techniques Used: Amplification, Incubation, Bacteria, Cell Culture

    S. hominis diminishes S. epidermidis- enhanced cytokine responses to UVR. NHEKs (p2–5) were incubated with media, S. hominis , S. epidermidis, or both for 30–60 min, media replaced with PBS, and subsequently exposed to UVB (0–20 mJ/cm²). Twenty-hours post-microbial inoculation, supernatants were collected and effects on cytokine secretion assessed by ELISA ( A–D ). **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.
    Figure Legend Snippet: S. hominis diminishes S. epidermidis- enhanced cytokine responses to UVR. NHEKs (p2–5) were incubated with media, S. hominis , S. epidermidis, or both for 30–60 min, media replaced with PBS, and subsequently exposed to UVB (0–20 mJ/cm²). Twenty-hours post-microbial inoculation, supernatants were collected and effects on cytokine secretion assessed by ELISA ( A–D ). **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.

    Techniques Used: Incubation, Enzyme-linked Immunosorbent Assay

    Related Articles

    Cell Culture:

    Article Title: Induction of the p53-target gene GADD45 in HPV-positive cancer cells.
    Article Snippet: .. Cell culture and genotoxic treatment All cells were grown in Dulbecco's minimal essential medium (pH 7.2), supplemented with fetal calf serum, with the exception of primary and E6-, E6/E7-, and E7immortalized human foreskin keratinocytes which were cultured in keratinocyte growth medium (Promocell, Heidelberg, Germany). .. For genotoxic treatment, exponentially growing cells were exposed to either 10 mg/ml mitomycin C (Sigma, Deisenhofen, Germany), 10 mg/ml cisplatin (ASTA Medica, Frankfurt, Germany), 50 J/m2 UV-irradiation (UV Stratalinker 2400, Stratagene, Heidelberg, Germany), or 20 gy g-irradiation employing a 137Cs source (Gammacell 1000, Atomic Energy of Canada Ltd., Canada), respectively.

    Article Title: Dipeptidyl peptidase IV (DP IV, CD26) is involved in regulation of DNA synthesis in human keratinocytes.
    Article Snippet: .. Commercially available human foreskin keratinocytes (NHEK, Clonetics) from adult and neonatal donors and primary human split-skin keratinocytes (RUM, ROL) from patients undergoing routine skin transplantation were cultured in serum-free keratinocyte growth medium (KGM, Promocell, Heidelberg). .. Pieces of split-skin obtained through routine skin transplantation were washed twice in Hank's balanced salt solution (HBSS, w/o Mg2 , Ca2 ) with HEPES 10 mM (HBSS, Boehringer Ingelheim, Germany), minced, placed dermal side down in 0.25% trypsin solution and left overnight at 43C.

    Article Title: 1,25-dihydroxyvitamin D3 regulates estrogen metabolism in cultured keratinocytes.
    Article Snippet: .. Purified human foreskin keratinocytes (Promocell, Heidelburg, Germany) were cultured in phenol red-free, serum-free, defined medium based on a modified MCDB 153 preparation with 28 mm HEPES buffer and containing the following additives: calcium chloride (0.15 mm), epidermal growth factor (recombinant human; 0.1 ng/ml), insulin (bovine; 5.0 mg/ml), hydrocortisone (0.5 mg/ml), gentamicin (50 mg/ml), and amphotericin B (2.5 mg/ml). ..

    Transplantation Assay:

    Article Title: Dipeptidyl peptidase IV (DP IV, CD26) is involved in regulation of DNA synthesis in human keratinocytes.
    Article Snippet: .. Commercially available human foreskin keratinocytes (NHEK, Clonetics) from adult and neonatal donors and primary human split-skin keratinocytes (RUM, ROL) from patients undergoing routine skin transplantation were cultured in serum-free keratinocyte growth medium (KGM, Promocell, Heidelberg). .. Pieces of split-skin obtained through routine skin transplantation were washed twice in Hank's balanced salt solution (HBSS, w/o Mg2 , Ca2 ) with HEPES 10 mM (HBSS, Boehringer Ingelheim, Germany), minced, placed dermal side down in 0.25% trypsin solution and left overnight at 43C.

    Purification:

    Article Title: 1,25-dihydroxyvitamin D3 regulates estrogen metabolism in cultured keratinocytes.
    Article Snippet: .. Purified human foreskin keratinocytes (Promocell, Heidelburg, Germany) were cultured in phenol red-free, serum-free, defined medium based on a modified MCDB 153 preparation with 28 mm HEPES buffer and containing the following additives: calcium chloride (0.15 mm), epidermal growth factor (recombinant human; 0.1 ng/ml), insulin (bovine; 5.0 mg/ml), hydrocortisone (0.5 mg/ml), gentamicin (50 mg/ml), and amphotericin B (2.5 mg/ml). ..

    Modification:

    Article Title: 1,25-dihydroxyvitamin D3 regulates estrogen metabolism in cultured keratinocytes.
    Article Snippet: .. Purified human foreskin keratinocytes (Promocell, Heidelburg, Germany) were cultured in phenol red-free, serum-free, defined medium based on a modified MCDB 153 preparation with 28 mm HEPES buffer and containing the following additives: calcium chloride (0.15 mm), epidermal growth factor (recombinant human; 0.1 ng/ml), insulin (bovine; 5.0 mg/ml), hydrocortisone (0.5 mg/ml), gentamicin (50 mg/ml), and amphotericin B (2.5 mg/ml). ..

    Recombinant:

    Article Title: 1,25-dihydroxyvitamin D3 regulates estrogen metabolism in cultured keratinocytes.
    Article Snippet: .. Purified human foreskin keratinocytes (Promocell, Heidelburg, Germany) were cultured in phenol red-free, serum-free, defined medium based on a modified MCDB 153 preparation with 28 mm HEPES buffer and containing the following additives: calcium chloride (0.15 mm), epidermal growth factor (recombinant human; 0.1 ng/ml), insulin (bovine; 5.0 mg/ml), hydrocortisone (0.5 mg/ml), gentamicin (50 mg/ml), and amphotericin B (2.5 mg/ml). ..



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    Dose-dependent effects on cytokine secretion. NHEKs (p3–5) cultured to 80% confluency were irradiated at 17–33 mJ/cm². Supernatants were collected 24 h post-irradiation and assessed via cytokine array ( A ) or via ELISA for IL-6 ( B ), IL-8 ( C ), CCL20 ( D ), and TNF-α ( E ). ELISA data are presented as mean ± SD. **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.

    Journal: Applied and Environmental Microbiology

    Article Title: Skin Staphylococcus species differentially modulate keratinocyte cytokine secretion in response to UVB

    doi: 10.1128/aem.01549-25

    Figure Lengend Snippet: Dose-dependent effects on cytokine secretion. NHEKs (p3–5) cultured to 80% confluency were irradiated at 17–33 mJ/cm². Supernatants were collected 24 h post-irradiation and assessed via cytokine array ( A ) or via ELISA for IL-6 ( B ), IL-8 ( C ), CCL20 ( D ), and TNF-α ( E ). ELISA data are presented as mean ± SD. **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.

    Article Snippet: Pooled ( n = 3), normal human epidermal keratinocytes (NHEKs from juvenile foreskin, Promocell C-12005; passages 1–5) were routinely cultured in keratinocyte growth medium (KGM-2, Promocell).

    Techniques: Cell Culture, Irradiation, Enzyme-linked Immunosorbent Assay

    Exposure to a skin PMC elevates cytokine secretion regardless of UVB exposure. NHEKs (p2–5) were treated with PBS (PBS, no UVB) or S. epidermidis, S. hominis, M. luteus, C. tuberculostearicum, and C. acnes for 30–90 min and subsequently exposed to UVB (33 mJ/cm²). Supernatants were collected 24 h post-inoculation with each organism, and the effects of the skin microbial PMC ± UVB on IL-6 ( A ), IL-8 ( B ), TNF-α ( C ), and CCL20 ( D ) secretion were assessed using ELISA. ** P < 0.01 and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.

    Journal: Applied and Environmental Microbiology

    Article Title: Skin Staphylococcus species differentially modulate keratinocyte cytokine secretion in response to UVB

    doi: 10.1128/aem.01549-25

    Figure Lengend Snippet: Exposure to a skin PMC elevates cytokine secretion regardless of UVB exposure. NHEKs (p2–5) were treated with PBS (PBS, no UVB) or S. epidermidis, S. hominis, M. luteus, C. tuberculostearicum, and C. acnes for 30–90 min and subsequently exposed to UVB (33 mJ/cm²). Supernatants were collected 24 h post-inoculation with each organism, and the effects of the skin microbial PMC ± UVB on IL-6 ( A ), IL-8 ( B ), TNF-α ( C ), and CCL20 ( D ) secretion were assessed using ELISA. ** P < 0.01 and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.

    Article Snippet: Pooled ( n = 3), normal human epidermal keratinocytes (NHEKs from juvenile foreskin, Promocell C-12005; passages 1–5) were routinely cultured in keratinocyte growth medium (KGM-2, Promocell).

    Techniques: Enzyme-linked Immunosorbent Assay

    UVB impacts the relative abundance of skin commensals in a polymicrobial skin community. NHEKs (p2–5) were incubated with a polymicrobial community for 90 min, media replaced, and cultures exposed to 33 mJ/cm2 UVB or left unirradiated (no UVB). Polymicrobial community composition was assessed 24 h post-treatment via qPCR, where relative proportions of each organism were calculated via species-specific standard curves and presented as collated data ( A , results for C. tuberculostearicum and M. luteus are not visible in the graph, as abundance was <1% of the total community) and UVB induced changes on individual species within the community of S. epidermidis ( B ), S. hominis ( C ), C. acnes ( D ), M. luteus ( E ), and C. tuberculostearicum ( F ). Data are presented as mean ± SD. ** P < 0.01 and * P < 0.05, as determined via unpaired t -test.

    Journal: Applied and Environmental Microbiology

    Article Title: Skin Staphylococcus species differentially modulate keratinocyte cytokine secretion in response to UVB

    doi: 10.1128/aem.01549-25

    Figure Lengend Snippet: UVB impacts the relative abundance of skin commensals in a polymicrobial skin community. NHEKs (p2–5) were incubated with a polymicrobial community for 90 min, media replaced, and cultures exposed to 33 mJ/cm2 UVB or left unirradiated (no UVB). Polymicrobial community composition was assessed 24 h post-treatment via qPCR, where relative proportions of each organism were calculated via species-specific standard curves and presented as collated data ( A , results for C. tuberculostearicum and M. luteus are not visible in the graph, as abundance was <1% of the total community) and UVB induced changes on individual species within the community of S. epidermidis ( B ), S. hominis ( C ), C. acnes ( D ), M. luteus ( E ), and C. tuberculostearicum ( F ). Data are presented as mean ± SD. ** P < 0.01 and * P < 0.05, as determined via unpaired t -test.

    Article Snippet: Pooled ( n = 3), normal human epidermal keratinocytes (NHEKs from juvenile foreskin, Promocell C-12005; passages 1–5) were routinely cultured in keratinocyte growth medium (KGM-2, Promocell).

    Techniques: Incubation

    UVB exacerbates S. epidermidis -induced cytokine secretion, while S. hominis amplified secretion of IL-8 and CCL20 regardless of UVB exposure. NHEKs (p2–5) were incubated with media alone, S. hominis, or S. epidermidis for 30–60 min, media replaced with PBS, and exposed to UVB (0–33 mJ/cm²). Adherent bacteria were then co-cultured with NHEKs for 24 h, following which supernatants were collected to assess the effects on IL-6 ( A and E ), CCL20 ( B and F ), IL-8 ( C and G ), and TNF-α ( D and H ). Significance was assessed via one-way ANOVA followed by Tukey test, where ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

    Journal: Applied and Environmental Microbiology

    Article Title: Skin Staphylococcus species differentially modulate keratinocyte cytokine secretion in response to UVB

    doi: 10.1128/aem.01549-25

    Figure Lengend Snippet: UVB exacerbates S. epidermidis -induced cytokine secretion, while S. hominis amplified secretion of IL-8 and CCL20 regardless of UVB exposure. NHEKs (p2–5) were incubated with media alone, S. hominis, or S. epidermidis for 30–60 min, media replaced with PBS, and exposed to UVB (0–33 mJ/cm²). Adherent bacteria were then co-cultured with NHEKs for 24 h, following which supernatants were collected to assess the effects on IL-6 ( A and E ), CCL20 ( B and F ), IL-8 ( C and G ), and TNF-α ( D and H ). Significance was assessed via one-way ANOVA followed by Tukey test, where ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

    Article Snippet: Pooled ( n = 3), normal human epidermal keratinocytes (NHEKs from juvenile foreskin, Promocell C-12005; passages 1–5) were routinely cultured in keratinocyte growth medium (KGM-2, Promocell).

    Techniques: Amplification, Incubation, Bacteria, Cell Culture

    S. hominis diminishes S. epidermidis- enhanced cytokine responses to UVR. NHEKs (p2–5) were incubated with media, S. hominis , S. epidermidis, or both for 30–60 min, media replaced with PBS, and subsequently exposed to UVB (0–20 mJ/cm²). Twenty-hours post-microbial inoculation, supernatants were collected and effects on cytokine secretion assessed by ELISA ( A–D ). **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.

    Journal: Applied and Environmental Microbiology

    Article Title: Skin Staphylococcus species differentially modulate keratinocyte cytokine secretion in response to UVB

    doi: 10.1128/aem.01549-25

    Figure Lengend Snippet: S. hominis diminishes S. epidermidis- enhanced cytokine responses to UVR. NHEKs (p2–5) were incubated with media, S. hominis , S. epidermidis, or both for 30–60 min, media replaced with PBS, and subsequently exposed to UVB (0–20 mJ/cm²). Twenty-hours post-microbial inoculation, supernatants were collected and effects on cytokine secretion assessed by ELISA ( A–D ). **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.

    Article Snippet: Pooled ( n = 3), normal human epidermal keratinocytes (NHEKs from juvenile foreskin, Promocell C-12005; passages 1–5) were routinely cultured in keratinocyte growth medium (KGM-2, Promocell).

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay